Recent Advances in Understanding Hydrophilic Interaction Chromatography (HILIC)
 Monday, March 3, 2008
8:30 a.m., Room 243

Organizer:

Michael McGinley, Phenomenex

Speakers:

8:35        Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection for Pharmaceutical Applications  DONALD RISLEY, Eli Lilly and Company

9:10       Application of a Structured Approach for Method Development in Bioanalytical Hydrophilic Interaction Liquid Chromatography with Mass Spectrometric Detection  CARL A SANCHEZ, Phenomenex, Monika Kansal, Art Dixon, Ismail Rustamov

9:45       Recess

10:00     Development and Optimization of HILIC-MS/MS Methods for Rapid GLP Bioanalysis  RAND G JENKINS, PPD, Inc., William Mylott

10:35     The Novelty of Zwitterionic Stationary Phases for Hydrophilic Interaction Liquid Chromatography (HILIC)  EINAR PONTEN, SeQuant AB

Overview:

With the increasing use of mass spectrometry detection for HPLC methods, hydrophilic interaction chromatography (HILIC) has seen a renewed interest as a separation method for polar compounds. HILIC HPLC methods elute polar compounds in a high organic mobile phase conducive to sensitive MS detection; this is in contrast to reversed-phase HPLC where retention and detection of highly polar compounds (typical of metabolites in ADME/ DMPK studies) can sometimes be challenging. While HILIC can be useful, there are many limitations that have previously confounded researchers. The limitations most cited include poor reproducibility and limited selectivity.

Recent advances due to improvements in separation methods as well new column technology have overcome many of the limitations that researchers had previously encountered with HILIC. The major leaders in the field of HILIC will discuss new techniques they have developed to improve both the selectivity and reproducibility of HILIC HPLC methods. In addition, different column manufacturers will discuss recent innovations in HILIC phase development that overcome many of the limitations previously encountered. Users will find many of the discussed insights key in developing improved HILIC methods.