The HY-LiTE Rapid Fuel Test is a quantitative bioluminescence test that indicates the presence of aerobic and anaerobic microorganisms in fuel and water associated with fuel by quantifying the amount of adenosine Triphosphate (ATP) present in the test sample. ATP is a unique biochemical that is responsible for energy metabolism and is present in all living organisms. The test is performed by preparing the sample as directed and then transferring a small volume of the sample to a test pen which combines the microbial ATP with other chemicals associated with the pen to activate the test en to produce light. The activated test pen is then inserted into an analyzer which quantifies and reports the light produced by the sample. The test is completed in less than 10 minutes and the results are directly proportional to the quantity of microbial ATP present in the sample.